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  • 多功能植物光合表型成像測量系統

多功能植物光合表型成像測量系統

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慧諾瑞德(北京)科技有限公司(PhenoTrait)是一家以植物表型為核心的AIoT+DT技術公司,是國家企業、中關村企業和全國科技型中小企業。公司利用智能感知、多源多維多譜視覺技術、人工智能、自動化和物聯網技術,為大范圍、高通量獲取與農作物品質、產量、抗性相關的植物表型及環境數據提供系統解決方案,為智慧育種、智慧種植和產業鏈賦能。表型組是基因組之后生命科學研究和產業應用的又一戰略制高點。慧諾瑞德,用表型之“瞳”,筑科研之基,拓產業之路,賦農業之慧。 公司是國際植物表型學會(IPPN)會員,創始人韓志國博士是IPPN執委會成員、工業分會副主席(2020-2024),也是我國“植物表型”這一細分市場的創建者。公司是亞太植物表型國際會議(APPPcon)發起單位和China Plant Phenotyping Network (CPPN)發起單位。公司先后榮登2020國際未來農業食品榜生物農業TOP20。和2022國際未來農業食品榜種業創新TOP20。 公司旗下的學術公眾號“植物表型資訊”,已成為華人植物表型圈影響力的公眾號;公司參與發起的“百博智慧大講堂”,已成為國內的線上學術講座平臺之一。
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多功能植物光合表型成像測量系統PlantExplorer采用創新的多光譜葉綠素熒光/可見光成像技術,利用的LED技術、CCD技術、通信技術,實現了對植物表型的創新測量,可以在獲取RGB成像、葉綠素成像、花青素成像的同時,獲取葉綠素熒光成像(成像面積53cmx53cm)
多功能植物光合表型成像測量系統 產品詳情

 

多功能植物光合表型成像測量系統PlantExplorer采用創新的多光譜葉綠素熒光/可見光成像技術,利用的LED技術、CCD技術、通信技術,實現了對植物表型的創新測量,可以在獲取RGB成像、葉綠素成像、花青素成像的同時,獲取葉綠素熒光成像(成像面積53cm x 53cm)。系統包括帶光學濾光輪的CCD成像系統、聚焦系統、嵌入式高亮度紅光LED、光譜白光LED、多光譜LED、嵌入式電腦和觸摸屏。由于采用一個CCD加濾光輪的組合,使得能夠在像素水平上進行圖像疊加計算。

 

多功能植物光合表型成像測量系統PlantExplorer包括三個版本:標準版PlantExplorer、高級版本PlantExplorerPro和適合高達120cm植物的版本PlantExplorerPro+。三個版本都可以選配GFP和/或RFP成像模塊(需在購買時指出,不可后續升級),其中PlantExplorer可以在購買后,再后續升級成PlantExplorerPro

 

 

功能特性

  • 創新的多功能植物光合表型平臺
  • 可見光成像+多光譜成像+葉綠素熒光(調制和非調制)成像
  • 同一個相機采集所有成像
  • 全自動馬達聚焦系統,帶全景和微距聚焦程序
  • 出色的高清相機(1.3 M pixel)測量葉綠素熒光
  • 高信噪比葉綠素熒光成像
  • 高質量10 Mp鏡頭,帶光譜可見光和近紅外涂層
  • 無可見鏡頭畸變,無需圖像校正
  • 濾光片可提供10個濾光片位置
  • 大景深設計
  • 成像范圍53 x 53cm
  • 可進行多光譜測量,精確獲知葉綠素熒光、葉綠素、花青素和R/G/B圖像每個像素的變化
  • 自動計算熒光參數和表型參數
  • 可設置進行延時成像測量
  • 嵌入式電腦進行精確的成像、時間控制、光強控制和數據存儲
  • 系統配置觸摸屏顯示器
  • 功能強大的控制和分析軟件

 

選購指南

 

  

主要技術參數

  • 相機傳感器類型:CCD
  • 相機分辨率:130萬像素
  • 圖像獲取時間:單張葉綠素熒光圖像20-1 000 us
  • 圖像格式:16位RAW格式
  • 光譜范圍:350~1000 nm
  • 激發光強度:25cm處,1500-6000 umol m-2 s-1;60cm處,800-3500 umol m-2 s-1。強度可調。
  • 光化光強度: 60cm處,100-600 umol m-2 s-1。強度可調。
  • 光學濾光片(適用于多光譜版):6種高質量光學干涉濾光片,包括熒光、紅光、綠光、藍光、花青素和近紅外濾光片
  • 成像面積:53 x 53 cm
  • 成像和計算的參數:Fo成像、Fm成像、Ft成像、Ft=5min成像、Fm’成像、Fv/Fm成像、Fq’成像、ΦPSII成像、ΦRO成像、NPQ100成像、qN成像、qP成像、Rfd100成像、 NDVI成像、RNIR成像、RChl成像.、RAnth成像、RRed成像、RGreen成像、RBlue成像、葉綠素指數成像、花青素指數成像和可見光成像,能夠自動計算投影葉面積、Fv/Fm平均值、低于Fv/Fm的面積百分比、ΦPSII平均值、低于ΦPSII的面積百分比、NPQ100平均值、高于NPQ100的面積百分比、Rfd100平均值、低于Rfd100的面積百分比、平均RGB比值、特殊RGB比值的面積百分比、平均葉綠素指數、低于葉綠素指數的面積百分比、平均花青素指數、低于花青素指數的面積百分比等(具體參數取決于版本),以及凸包、最小外接圓、最小外接矩形等相關表型參數。

 

應用舉例

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

利用PhenoVation光合表型成像技術發表的部分文獻

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  3. Farooq M, van Dijk A D J, Nijveen H, et al. (2021) Prior Biological Knowledge Improves Genomic Prediction of Growth-Related Traits in Arabidopsis thaliana. Frontiers in Genetics, 11:609117. doi: 10.3389/fgene.2020.609117
  4. He Y, Li Y, Yao Y et al. (2021) Overexpression of watermelon m6A methyltransferase ClMTB enhances drought tolerance in tobacco by mitigating oxidative stress and photosynthesis inhibition and modulating stress-responsive gene expression. Plant Physiology and Biochemistry, 168: 340-352.
  5. Wang W, Liu D, Qin M et al. (2021) Effects of Supplemental Lighting on Potassium Transport and Fruit Coloring of Tomatoes Grown in Hydroponics. International Journal of Molecular Sciences, 22(5): 2687 https://doi.org/10.3390/ijms
  6. Singh R R, Pajar J A, Audenaert K, et al. (2021) Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes. Frontiers in Plant Science, 12:713870. doi: 10.3389/fpls.2021.713870
  7. Vidak M, Lazarevic B, Petek M, et al. (2021) Multispectral Assessment of Sweet Pepper (Capsicum annuum L.) Fruit Quality Affected by Calcite Nanoparticles. Biomolecules, 11(6), 832; https://doi.org/10.3390/biom
  8. Lazarevic B, Satovic Z, Nimac A, et al. (2021) Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.). Frontiers in Plant Science, 12:629441. doi: 10.3389/fpls.2021.629441
  9. Romero-Perez A, Ameye M, Audenaert K, et al. (2021) Overexpression of F-Box Nictaba Promotes Defense and Anthocyanin Accumulation in Arabidopsis thaliana After Pseudomonas syringae Infection. Frontiers in Plant Science, 12:692606. doi: 10.3389/fpls.2021.692606
  10. Meng L, Mestdagh H, Ameye M, et al. (2021) Phenotypic variation of Botrytis cinerea Isolates is influenced by spectral light quality. Frontiers in Plant Science, 11:1233. doi: 10.3389/fpls.2020.01233
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  12. Stambuk P, Sikuten I, Preiner D, et al. (2021) Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging. Plants, 10, 661. https://doi.org/10.3390/plants
  13. Tan J, de Zutter N, de Saeger S, et al. (2021) Presence of the Weakly Pathogenic Fusarium poae in the Fusarium Head Blight Disease Complex Hampers Biocontrol and Chemical Control of the Virulent Fusarium graminearum Pathogen. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.641890
  14. Flood P, Theeuwen T, Schneeberger K, Keizer P, Kruijer W, et al. (2020) Reciprocal cybrids reveal how organellar genomes affect plant phenotypes. Nature Plants, 10.1038/s41477-019-0575-9ff. ffhal-v2f
  15. Velivelli S L S, Czymmek K J, Li H, Shaw J B, Buchko G W, Shah D M. (2020) Antifungal symbiotic peptide NCR044 exhibits unique structure and multifaceted mechanisms of action that confer plant protection. PNAS, DOI: 10.1073/pnas.2003526117
  16. Bhatnagar N, Pandey S. (2020) Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants. Plant Physiology, DOI: https://doi.org/10.1104/pp.20.01309
  17. Venneman J, Vandermeersch L, Walgraeve C et al. (2020) Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2020.544435
  18. Tan J, Ameye M, Landschoot S et al. (2020) At the scene of the crime: New insights into the role of weakly pathogenic members of the fusarium head blight disease complex. Molecular Plant Pathology, DOI: 10.1111/mpp.12996
  19. Prinzenberg A E, Campos-Dominguez L, Kruijer W, Harbinson J, Aarts M G M. (2020) Natural variation of photosynthetic efficiency in Arabidopsis thaliana accessions under low temperature conditions. Plant Cell & Environment, 1–14. https://doi.org/10.1111/pce.13811
  20. Zhang H, Chen Y, Niu Y, Zhang X, Zhao J, Sun L, Wang H, Xiao J, Wang X. (2020) Characterization and fine mapping of a leaf yellowing mutant in common wheat. Plant Growth Regulation, https://doi.org/10.1007/s10725-020-00633-0
  21. Jin X, Zarco-Tejada P, Schmidhalter U, Reynolds M P et al. (2020) High-throughput estimation of crop traits: A review of ground and aerial phenotyping platforms. IEEE Geoscience and Remote Sensing Magazine, DOI: 10.1109/MGRS.2020.2998816
  22. Sheng X-G, Branca F, Zhao Z-Q et al. (2020) Identification of Black Rot Resistance in a Wild Brassica Species and Its Potential Transferability to Cauliflower. Argonomy, 10: 1400. doi:10.3390/agronomy
  23. Pennisi G, Blasioli S, Cellini A, Maia L, Crepaldi A, Braschi I, Gianquinto G. (2019). Unraveling the Role of Red:Blue LED Lights on Resource Use Efficiency and Nutritional Properties of Indoor Grown Sweet Basil. Frontiers in plant science, 10, 305. doi:10.3389/fpls.2019.00305
  24. Pennisi G, Orsini F, Blasioli S, Cellini A et al. (2019) Resource use efficiency of indoor lettuce (Lactuca sativa L.) c*tion as affected by red:blue ratio provided by LED lighting. Scientific Reports, 9, 14127
  25. Van Es S W, van der Auweraert E B, Silveira S R, Angenent G C, van Dijk A D J, Immink R G H. (2019) Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination. The Plant Journal, doi: 10.1111/tpj.14326
  26. Köhl J, Goossen-van de Geijn H, Groenenboom-de Haas L, et al. (2019) Stepwise screening of candidate antagonists for biological control of Blumeria graminis f. sp. tritici. Biological Control, 136: 104008
  27. Mohd Nadzir M M, Vieira Lelis F M, Thapa B, Ali A, Visser R G F, van Heusden A W, van der Wolf J M. (2019) Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species. Plant Phathology, 68(1): 42-48
  28. Sall K, Dekkers B J W, Nonogaki M, Katsuragawa Y, Koyari R, Hendrix D, Willems L A J, Bentsink L, Nonogaki H. (2019) DELAY OF GERMINATION  1LIKE  4 acts as an inducer of seed reserve accumulation. The Plant Journal, 100: 7-19.
  29. Li H, Velivelli S L S, Shah D M. (2019) Antifungal Potency and Modes of Action of a Novel Olive Tree Defensin Against Closely Related Ascomycete Fungal Pathogens. Molecular Plant-Microbe Interactions. 32(12): 1646-1664.
  30. Prinzenberg A E, Viquez-Zamora M, Harbinson J, Lindhout P, van Heusden S. (2018) Chlorophyll fluorescence imaging reveals genetic variationand loci for a photosynthetic trait in diploid potato. Physiologia Plantarum, 164: 163-175.
  31. Van Rooijen R, Harbinson J, Aarts M G M. (2018) Photosynthetic response to increased irradiance correlates to variation in transcriptional response of lipidremodeling and heatshock genes. Plant Direct, 2(7): e00069
  32. Van Bezouw R F H M, Keurentjes J J B, Harbinson J, Aarts M G. (2018) Converging phenomics and genomics to study natural variation in plant photosynthetic efficiency. Plant Journal, 97(1): 112-133.
  33. Domazakis E, Wouters D, Visser R G F, Kamoun S, Joosten M H A J, Vleeshouwers V G A A. (2018) The ELR-SOBIR1 Complex Functions as a Two-Component Receptor-Like Kinase to Mount Defense Against Phytophthora infestans. Molecular Plant-Microbe Interactions, 31(8): 795-802.
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  35. Van Rooijen R, Kruijer W, Boesten R, van Eeuwijk F A, Harbinson J, Aarts M G M. (2017) Natural variation of YELLOW SEEDLING1 affects photosynthetic acclimation of Arabidopsis thaliana. Nature Communications, 8: 1421
  36. Flood P J, Kruijer W, Schnabel S K, van der Schoor R, Jalink H, Snel J F H, Harbinson J, Aarts M G M. (2016) Phenomics for photosynthesis, growth and reflectance in Arabidopsis thaliana reveals circadian and long-term fluctuations in heritability. Plant Methods, 12: 14. https://doi.org/10.1186/s13007-016-0113-y
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